Laboratory of Cancer Treatment and Tissue Regeneration

Václav Liška et al. at the Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Czech Republic

Lab Profile

The Laboratory of Cancer Treatment and Tissue Regeneration, led by Assoc. prof. Václav Liška, M.D., Ph.D., is one of the laboratories of the Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Czech Republic.

The main research focus of the lab is regeneration of parenchymal organs in relation to extensive surgical procedures. Our goal is to develop and/or experimentally verify all possibilities to improve the surgical treatment of patients with cancers, especially liver malignancies. The core of our work is experimental surgery in large animal models, which is supplemented by closely interconnected research of imaging methods, organ structure and perfusion, and clinical aspects of cancer treatment as well as by development of in-house image analysis and mathematical modelling software. We also focus on tissue engineering strategies to evaluate the use of a porcine-derived scaffold (obtained via decellularization) as matrix to be functionalized in vitro with in-house developed bioreactors.

The Research

In our experimental surgery projects, we have established (in domestic pig) experimental models of steatohepatitis associated with chemotherapy, toxic alcoholic liver disease, and biliary cirrhosis.

We also develop software for advanced image analysis to predict regeneration capacity of the liver parenchyma and diagnose focal and diffuse liver diseases. Computational modelling features developed in collaboration with the Faculty of Applied Sciences, University of West Bohemia, Pilsen, are being continuously implemented in the software.

We also focus on imaging of portal system microcirculation and we explore the possibilities to apply our findings in clinical practice.

We are exploring the possibility to use decellularized porcine tissue as scaffold for the in vitro functionalization with human cells in our own bioreactors for tissue engineering.

We closely cooperate with the Labs of Pharmacogenomics, Translational Cancer Genomics, Tumor Biology and Quantitative Histology and we try to transfer their research results to our clinical requirements and applications.

Long-term research aims

  • regeneration of liver parenchyma and liver surgery

  • liver and other tissue decellularization for regenerative medicine

  • portal micro- and macrocirculation

  • reconstruction of portal system

  • surgical and oncological treatment of malignancies

  • immune system behaviour during progression of tumour disease

The Summer School

Each summer, the team of the Laboratory of Cancer Treatment and Tissue Regeneration organise the international Summer School of Experimental Surgery for pre-graduate medical students.

The course lasts two weeks and aims not only to teach the students surgical techniques and procedures in extensive practical exercises, but also to introduce them to the possibilities, limits and boundaries of the field as well as to the related areas of biomedical research. The for the purposes Summer School, we also run moderately popular Facebook page and Youtube channel.

The Team

(selected members' names are hyperlinked to their respective ORCID profiles)

Main team members

  • Vladimíra Moulisová, M.Sc., Ph.D., biochemist

  • Jan Brůha, M.D., Ph.D., surgeon

  • Richard Pálek, M.D., Ph.D., surgeon

  • Jáchym Rosendorf, M.D., Ph.D., surgeon

  • Ondřej Vyčítal, M.D., Ph.D., surgeon

  • Petr Hošek, Ph.D., statistician, biomedical engineer

  • Miroslav Jiřík, M.Sc., Ph.D., software engineer

  • Lenka Červenková, MVDr., PhD student, phatologist

  • Maria Stefania Massaro , M.Sc., PhD student, biomedical engineer

  • Robert Polák, M.D., PhD student, surgeon

  • Sima Šarčević, M.D., PhD student, surgeon

  • Roshan Kumar Singh, M.Sc., PhD student

  • Zuzana Fišerová, assistant

  • Petra Havlíčková, B.Sc., assistant

  • Lenka Kuberková, assistant

  • Nela Štorková, Ph.D., M.Sc., assistant

  • Marek Brousil, medical student

  • Ondřej Brzoň, B.Sc., medical student

  • Táňa Drhovská, medical student

  • Lucie Kepková, medical student

  • Filip Kout, medical student

  • Pavel Krystl, medical student

  • Jan Ševčík, medical student

  • Lukáš Plzák, medical student

  • Tomaš Polenda, medical student

  • Daniel Vydra, medical student

Main collaborators from other faculty departments

  • Jiří Škorpil, M.D., Ph.D., University Hospital Pilsen

  • Jan Benes, M.D., Ph.D., Department of Anaesthesiology and Resuscitation

  • Lada Eberlová, M.D., Ph.D., Department of Anatomy

  • Lukáš Bolek, M.D., Ph.D., Department of Biophysics

  • Jiří Dejmek, M.Sc., Ph.D., Department of Biophysics

  • Martina Grajciarová, MVDr., Department of Histology and Embryology

  • Anna Malečková, M.D., Department of Histology and Embryology

  • Zbynek Tonar, M.D., M.Sc., Ph.D., Department of Histology and Embryology

  • Jitka Černohousová, M.Sc., M.A., Department of International Relations

  • Kristýna Bajcurová, M.D., Ph.D., Department of Imaging Methods

  • Eva Korčáková, M.D., Ph.D., Department of Imaging Methods

  • Hynek Mírka, M.D., Ph.D., Department of Imaging Methods

  • Jaroslav Hrabak, M.Sc., Ph.D., Department of Microbiology

  • Ondřej Fiala, M.D., Ph.D., Department of Oncology

  • Milan Stengl, M.D., Ph.D., Department of Physiology

  • Radka Václavíková, M.Sc., Ph.D., Laboratory of Pharmacogenomics

  • Simona Šůsová, M.Sc., Laboratory of Pharmacogenomics

  • Tomas Sychra, M.D., Laboratory of Pharmacogenomics

  • Filip Ambrozkiewicz, M.D., Ph.D., Laboratory of Translational Cancer Genomics

  • Andriy Trailin, M.D., D.Sc., Ph.D., Laboratory of Translational Cancer Genomics

The Book

We have also created the book Experimental Surgery, which serves as a textbook for the Summer School, but we believe that it can be useful for many medical students and Ph.D. students interested in surgery and biomedical research in general.

In 2018, the book was awarded the Maydl Prize of the Czech Medical Association of J. E. Purkyně.

Liska V. (ed.) et al. Experimental Surgery. Pilsen: Nava, 2016. ISBN 978-80-7211-490-0.